Abstracts

List of abstracts confirmed by the authors for publication on the conference website.

S 1-2
Macrophage screen identifies Francisella genes required for intracellular replication
A. Llewellyn¹, B. Napier¹, D. Weiss^1
1Emory University, Emory Vaccine Center, Atlanta, United States

S 1-3
Determining the function of the cytoplasmic membrane protein RipA
B. Mortensen¹, J. Fuller¹, M. Huang¹, T. Kijek¹, C. Miller¹, S. Taft-Benz¹, T. Kawula^1
1University of North Carolina, Microbiology and Immunology, Chapel Hill, United States

S 2-1
Intracellular nutrition and F. tularensis pathogenesis
A. Charbit^1
1INSERM U570, Université Paris Descartes, Faculté de Médecine Necker-Enfants Malades, Paris, France

S 2-3
Type six secretion in Francisella
F. Nano^1
1University of Victoria, Biochemistry and Microbiology, Victoria, Canada

S 2-4
Role of Type IV pilin genes in virulence of Francisella tularensis subspecies holartica and tularensis
A.-L. Forslund^1,2, E. Salomonsson¹, K. Kuoppa¹, I. Golovliov³, S. Michell⁴, R. Titball⁴, P. Oyston⁵, L. Noppa¹, A. Sjöstedt³, Å. Forsberg^1,2
1FOI Swedish Defence Research Agency, Department of CBRN Defence and Security, Umeå, Sweden, ²Umeå University, Department of Molecular Biology, Umeå, Sweden, ³Umeå University, Umeå Centre for Microbial Research (UCMR) and Laboratory for Molecular Infection Medicine (MIMS, Umeå, Sweden, ⁴University of Exeter, School of Biosciences, Devon, United Kingdom, 5Porton Down, Defence Science and Technology Laboratory, Salisbury, United Kingdom

S 3-4
A genome-scale phenotype map of Francisella novicida
M. Enstrom¹, K. Held¹, C. Manoil^1
1University of Washington, Genome Sciences, Seattle, United States

S 4-1
Heterologous expression of Francisella tularensis type IV pili genes in Neisseria gonorrhoeae confirms that PilA can form functional pili
E. Salomonsson^1,2_, Å. Forsberg^1,2, N. Roos³, C. Holz⁴, B. Maier⁴, M. Koomey^3,5, H.C. Winther-Larsen^3,5
1FOI - CBRN Defence and Security, Umeå, Sweden, ²Umeå University, Department of Molecular Biology, Umeå, Sweden, ³University of Oslo, Department of Molecular Biosciences, Oslo, Norway, ⁴Westfälische Wilhelms-Universität Münster, Institut für Allgemeine Zoologie und Genetik, Münster, Germany, ⁵University of Oslo, Centre for Molecular Biology and Neuroscience, Oslo, Norway

S 4-2
Identification of F. tularensis from environmental water specimens in tularemia epidemics in Turkey by both culture and real time TaqMan PCR methods
H. Simsek¹, M. Taner¹, A. Karadenizli², M. Ertek¹, H. Vahaboglu^3
1Refik Saydam National Institute of Hygiene, Ankara, Turkey, ²Kocaeli University Medical Faculty, Microbiology & Clinical Microbiology, Kocaeli, Turkey, ³Kocaeli University Medical Faculty, Infectious Disease, Kocaeli, Turkey

S 4-3
Characterization of a novel Francisella sp. from blood and urine of a patient with an unusual clinical presentation
R. Escudero¹, M. Elía², V. Menéndez³, J.A. Sáez-Nieto¹, L. Herrera¹, J.A. Galán³, M. Ruiz², G. Rollo², I. Jado¹, H. Gil¹, M. Rodríguez-Vargas¹, P. Anda^1
1National Center of Microbiology. ISCIII, Bacteriology, Majadahonda, Madrid, Spain, ²General University Hospital, Microbiology, Elche, Alicante, Spain, ³General University Hospital, Urology, Elche, Alicante, Spain

S 4-4
Description of ´Francisella novicida´ FSC454T as a novel species of the genus Francisella
B. Huber¹, H.-J. Busse¹, E. Seibold², H.C. Scholz², P. Kämpfer³, W.D. Splettstoesser^2
1Inst. of Bacteriology, Mycology & Hygiene, Vienna, Austria, ²Bundeswehr Institute of Microbiology, Munich, Germany, ³Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Giessen, Germany

S 6-3
Interaction of mosquito larvae with Francisella tularensis LVS biofilms
U. Mahajan¹, M.W. Turnbull¹, T.L. McNealy^1
1Clemson University, Biological Sciences, Clemson, United States

S 8-1
Fate of the complex formed between nucleolin present on membrane of human cells and LVS surface elongation factor Tu (EF-Tu) during LVS infection
M. Barel¹, K. Meibom¹, A. Charbit^1
1Université Paris V René Descartes, INSERM U570, Paris, France

S 8-2
Differences in virulence between subspecies of Francisella tularensis are reflected in different gene expression profiles of human neutrophils
E. Seibold¹, M. Bastian², N. Knauer¹, D. Koczan², D. Frangoulidis¹, W. Splettstoesser¹, P. Schuff-Werner^2
1Bundeswehr Institute of Microbiology, Department of Immunology and Epidemiology, Munich, Germany, ²Institute of Clinical Chemistry & Laboratory Medicine, Medical Faculty, University Rostock, Germany

S 8-5
The 58-kDa major virulence factor, FTT0918, of Francisella tularensis is required for utilization of iron
H. Lindgren¹, M. Honn¹, I. Golovlev¹, K. Kadzhaev¹, W. Conlan², A. Sjöstedt^1
1Umeå University, Clinical Bacteriology, Umeå, Sweden, ²National Research Council Canada, Institute for Biological Sciences, Ottawa, Canada

S 9-3
Integrating Francisella’s intracellular lifestyle
S. Daefler
 
S 9-4
Mast cell-mediated signaling in reduction of Francisella tularensis Schu S4 intramacrophage replication and apoptosis
A.R. Rodriguez¹, J.-J. Yu¹, J.P. Chambers¹, K.E. Klose¹, M.N. Guentzel¹, M.T. Berton², B.P. Arulanandam^1
1University of Texas at San Antonio, South Texas Center for Emerging Infectious Diseases, Biology Department, San Antonio, United States, ²University of Texas Health Science Center at San Antonio, Microbiology Department, San Antonio, United States

S 10-5
Quantitative proteomic profiling of host - pathogen interaction: The interaction of Francisella tularensis LVS with macrophage using J774.2 cell line
A. Hartlova¹, M. Link², J. Lenco², J. Stulik^2
1University of Defence, Faculty of Military Health Sciences, Centre of Advanced Studies, Hradec Kralove, Czech Republic, ²University of Defence, Faculty of Military Health Sciences, Institute of Molecular Pathology, Hradec Kralove, Czech Republic

S 11-1
Humoral immunity to Francisella tularensis strain LVS fails to uniformly protect Cynomolgus macaques from disease induced by aerosol infection with strain SCHU S4
J.A. Wilder¹, M. Valderas¹, A. Monier¹, T. Brasel¹, R. Sherwood¹, C.R. Lyons^2
1Lovelace Respiratory Research Institute, Albuquerque, United States, ²Universtiy of New Mexico, Albuquerque, United States

S 11-2
Exposure of cynomolgus macaques to aerosolized Francisella tularensis SCHU S4
H.C. Gelhaus¹, D. Pak¹, A. Lindsay¹, J. Cross¹, J. Hying¹, K. Battles¹, R. Krile¹, R. Barnewall¹, K. Van Zandt¹, H.A. Lockman^1
1Battelle Biomedical Reseach Center, Columbus, United States

S 11-3
Comparison of aerosolized F. tularensis in three species of nonhuman primates
A. Glynn¹, D. Alves², R. Erwin-Cohen¹, O. Espenshade¹, D. Waag³, A. Nalca^1
1U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Center for Aerobiological Sciences, Frederick, United States, ²USAMRIID, Pathology Division, Frederick, United States, ³USAMRIID, Bacteriology Division, Frederick, United States

S 12-1
Novel live vaccine candidates against airborne Francisella tularensis
J.W. Conlan¹, H. Shen¹, I. Golovliov², C. Zingmark², S. Twine¹, R. Claudine¹, P.C.F. Oyston³, C. Wangxue¹, R.V. House⁴, A. Sjostedt^2
1National Research Council Canada, Ottawa, Canada, ²Umeå University, Umea, Sweden, ³dstl Porton Down, Salisbury, United Kingdom, ⁴DynPort Vaccine Company LLC, Frederick, United States

S 12-2
Type A F. tularensis induces caspase-3-dependent macrophage death in infected tissues
J.R. Wickstrum¹, S.M. Bokhari¹, J.L. Fischer¹, D.M. Pinson², H.W. Yeh³, R.T. Horvat², M.J. Parmely^1
1University of Kansas Medical Center, Microbiology, Kansas City, United States, ²University of Kansas Medical Center, Pathology and Laboratory Medicine, Kansas City, United States, ³University of Kansas Medical Center, Biostatistics, Kansas City, United States

S 12-3
Molecular mechanisms responsible for antibody-mediated clearance of F. tularensis
D.W. Metzger¹, T. Smith Alumni^1
1Center for Immunology and Microbial Disease, Albany Medical College, Albany, United States

S 12-4
Essential elements of protective immunity to Francisella
K.L. Elkins^1
1Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U.S. FDA, Bethesda, United States

S 12-5
Derivation of a panel of potential correlates of vaccine-induced protection against Francisella tularensis LVS
R. De Pascalis¹, A.Y. Chou¹, K.L. Elkins^1
1Food and Drug Administration, Bethesda, United States

P1-01
A constraints-based systems approach to metabolic analysis of Francisella tularensis during infection
A. Raghunathan¹, S. Shin¹, S. Daefler^1
1Mount Sinai School of Medicine, Infectious Diseases, New York, United States

P1-03
Francisella novicida as a model to study tick transmission
S.M. Noh¹, C. Manoil², K.A. Brayton³, G.A. Scoles¹, G.H. Palmer^4
1USDA-Agriculture Research Service, Animal Disease Research Unit, Pullman, United States, ²University of Washington, Department of Genome Sciences, Seattle, United States, ³Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, United States, ⁴Washington State University, School for Global Animal Health, Pullman, United States

P1-04
Molecular typing of Francisella tularensis strains isolated in Georgia
G. Chanturia¹, E. Zhgenti¹, M. Zakalashvili¹, M. Kekelidze¹, E. Garcia², V.H.I. Lao², J. Elliott², P. Chain², N. Tsertsvadze³, L. Bakanidze³, P. Imnadze^4
1National Center for Disease Control and Public Health, Molecular Epidemiology, Tbilisi, Georgia, ²Lawrence Livermore National Lab, Biosciences & BioTechnology Division, Chemistry, Materials, Earth and Life Sciences Directorate, Livermore, United States, ³National Center for Disease Control and Public Health, Especially Dangerous Pathogens Department, Tbilisi, Georgia, ⁴National Center for Disease Control and Public Health, Tbilisi, Georgia

P1-05
Phenotypical analysis of the putative 2nd polysaccharide gene cluster of Francisella tularensis
R.M. Thomas¹, R.W. Titball², S.M. Twine³, P.C. Oyston¹, S.L. Michell², J.L. Prior^1
1Dstl Porton Down, Salisbury, United Kingdom, ²University of Exeter, School of Biosciences, Exeter, United Kingdom, ³NRC-Institute for Biological Sciences, Ottawa, Canada

P1-06
Effect of Hfq on Francisella tularensis growth and stress resistance
J.R. Chambers¹, K.S. Bender^1
1Southern Illinois University, Microbiology, Carbondale, United States

P1-08
Production of outer membrane vesicles and tube-like structures by Francisella tularensis
W.D. McCaig¹, D.G. Thanassi^1
1Stony Brook University, Center for Infectious Diseases Department of Molecular Genetics and Microbiology, Stony Brook, United States

P1-09
The phosphoproteome of pathogenic bacterium Francisella tularensis
P. Spidlova¹, L. Hernychova¹, J. Stulik^1
1University of Defence, Faculty of Military Health Sciences, Institute of Molecular Pathology, Hradec Kralove, Czech Republic

P1-10
Comparative proteome analysis of Francisella tularensis LVS and DsbA deletion mutant: identification of potential substrate proteins for the DsbA
I. Pavkova¹, M. Link¹, J. Stulik^1
1Faculty of Military Health Science, University of Defence, Institute of Molecular Pathology, Hradec Kralove, Czech Republic

P1-11
Analysis of the phenotype associated with the disruption of mglA in Fransicella tularensis LVS underlines its central role in manifestation of virulence
G. Zaide¹, E. Bar-Haim¹, I. Mendelson¹, O. Gat¹, S. Ehrlich¹, G. Friedman¹, O. Cohen¹, A. Shafferman^1
1Israel Institue for Biological Research, Biochemistry and Molecular Genetics, Ness-Ziona, Israel

P1-12
Mapping the presence of Francisella tularensis glycoproteins
L. Balonova^1,2,3, L. Hernychova¹, Z. Bilkova³, M.V. Novotny⁴, J. Stulik^1
1University of Defence, Institute of Molecular Pathology, Hradec Kralove, Czech Republic, ²University of Pardubice, Department of Analytical Chemistry, Pardubice, Czech Republic, ³University of Pardubice, Department of Biological and Biochemical Sciences, Pardubice, Czech Republic, ⁴Indiana University, Department of Chemistry, Bloomington, United States

P1-13
The only alternative s factor of Francisella tularensis is a genuine heat shock s factor
N. Grall¹, J. Livny², M. Waldor^2,3, M. Barel¹, A. Charbit¹, K.L. Meibom^1
1Université Paris V René Descartes, INSERM U570, Paris, France, ²Harvard Medical School, Channing Laboratories, Brigham and Women’s Hospital, Boston, United States, ³Howard Hughes Medical Institute, Boston, United States

P1-14
Genotyping of the Health Protection Agency Francisella strain collection
J. Mason¹, J. Latham¹, A. Sweed¹, S. Welch¹, K. Pearson¹, J. Burton¹, R. Vipond¹, A. Pearson^2
1Health Protection Agency, Salisbury, United Kingdom, ²Health Protection Agency, Centre for Infections, London, United Kingdom

P1-18
Non-antibiotic selectable markers for Francisella tularensis strains LVS and SchuS4
S.A. Smith¹, F.G. White¹, A. Lair¹, K. Moore¹, S. Soni², J.S. Gunn^2
1Battelle Memorial Institute, Columbus, United States, ²The Ohio State University, Columbus, United States

P1-21
Results from screening the F. novicida transposon two-allele mutant library
X.-H. Lai¹, L. Gallagher², C. Manoil², F. Heffron^1
1Oregon Health Science Univeristy, Portland, United States, ²University of Washington, Seattle, United States

P1-22
Identification of Francisella loci that impact expression of ripA
T. Kijek¹, J. Fuller¹, B. Mortensen¹, S. Taft-Benz¹, T. Kawula^1
1University of North Carolina, Microbiology and Immunology, Chapel Hill, United States

P1-24
Siderophore utilization in Francisella tularensis Live Vaccine Strain (LVS)
G. Ramakrishnan¹, A. Meeker^1
1University of Virginia, Medicine/Division of Infectious Diseases, Charlottesville, United States

P1-28
Type IV pilin-like proteins of Francisella tularensis contribute to virulence in a context-dependent manner
N.M. Ark¹, B.J. Mann^1
1University of Virginia, Charlottesville, United States

P1-34
Validation of Etest® for the determination of antibiotic susceptibilities of Francisella tularensis
E. Valade¹, J. Vaissaire², A. Mérens³, F.M. Thibault¹, D.R. Vidal¹, J.-D. Cavallo^3
1IRBA/CRSSA, La Tronche, France, ²Académie Vétérinaire de France, Paris, France, ³Hopital d'Instruction des Armées Bégin, Saint-Mandé, France

P1-35
Tularemia wet-lab exercise
M. Forsman¹, J. Petersen², M. Chu³, R. Grunow⁴, A. Johansson^5
1Sweden Defence Research Agency, Umeå, Sweden, ²Centers for Disease Control and Prevention, Fort Collins, United States, ³World Health Organization, Geneva, Switzerland, ⁴Robert Koch-Institut, Berlin, Germany, ⁵Umeå University Hospital, Umeå, Sweden

P1-36
Development of reference material to perform EU- wide proficiency tests for the diagnosis of highly pathogenic bacteria, including Francisella tularensis
U. Sauer¹, D. Jacob¹, R. Grunow^1
1Robert Koch Institut, ZBS 2, Berlin, Germany

P1-39
Therapy approach and problems in treatment tularemic patients in Serbia
M.M. Djordjevic¹, V. Kostic¹, M. Krstic¹, B. Lako^2
1Clinical Center University of Nis, Infectious Diseases Clinic, Nis, Serbia, ²Agriculture Faculty University of Novi Sad, Department for Veterinary Medicine, Novi Sad, Serbia

P1-40
Detection of Francisella tularensis in the European hare by real-time TaqMan PCR
N. MADANI¹, C. Mendy¹, M. El Khoury², B. Durand³, B. Garin-Bastuji^1
1French Food Safety Agency (AFSSA), Bacterial Zoonoses Unit, Animal Diseases & Zoonoses Research Laboratory, Maisons-Alfort, France, ²Hôpital Hôtel Dieu, Service Hématologie Biologique, Laboratoire de Cytogénétique, Paris, France, ³French Food Safety Agency (AFSSA), Epidemiology Unit, Maisons-Alfort, France

P1-42
Prolonged course of tick-borne ulceroglandular tularemia in a 20-year-old patient in Germany - case report
C. Luebbert¹, C. Taege², T. Seufferlein¹, R. Grunow^3
1Martin Luther University Halle-Wittenberg, University Hospital, First Department of Medicine, Halle (Saale), Germany, ²Institute for Pathology, Lutherstadt Eisleben, Germany, ³Robert Koch Institute, Centre for Biological Safety, Berlin, Germany

P1-43
First isolation of Francisella tularensis subspecies holarctica from foxes (Vulpes vulpes) in Germany
H. R. Nattermann¹, D. Jacob¹, S. Becker¹, A. Kühn¹, P. Kutzer², P. Lochau¹, C. Schulze², R. Grunow^1
1Robert Koch-Institute, Centre for Biological Safety (ZBS 2), Berlin, Germany, ²Landeslabor Berlin-Brandenburg, Fb III-1 Pathologie, Bakteriologie, Frankfurt (Oder), Germany<//strong>

P1-45
Enhanced growth of Francisella tularensis in a liquid nutrient medium (medium T)
D. Jacob¹, H.R. Nattermann¹, R. Krüger¹, S. Becker¹, R. Grunow^1
1Robert Koch-Institut, Centre for Biological Safety (ZBS 2), Berlin, Germany

P1-46
Prevalence of Francisella tularensis in European brown hare populations in Lower Saxony, Germany
M. Runge¹, M. von Keyserlingk¹, S. Braune¹, M. Wedekind², A. Grauer², U. Voigt², W. Splettstoesser³, P. Otto⁴, W. Müller⁴, K. Pohlmeyer^2
1LAVES - Veterinary Institute Hannover, Hannover, Germany, ²Institute for Wildlife Research, University of Veterinary Medicine Hannover, Foundation (IWFo), Hannover, Germany, ³Bundeswehr Institute of Microbiology, Munic, Germany, ⁴Federal Research Institute for Animal Health, Friedrich-Loeffler-Institute (FLI), Jena, Germany

P2-01
Francisella Sec secretion contributes to attachment during biofilm formation and chitin colonization
J.J. Margolis¹, S. El-Etr², L.-M. Joubert³, E. Moore⁴, R. Robison⁴, A. Rasley², A. Spormann⁵, D.M. Monack^1
1Stanford University, Department of Microbiology & Immunology, Stanford, United States, ²Lawrence Livermore National Lab, Biosciences and Biotechnology Division, Livermore, United States, ³Stanford University, Cell Sciences Imaging Facility, Stanford, United States, ⁴Brigham Young University, Department of Microbiology and Molecular Biology, Provo, United States, ⁵Stanford University, Department of Civil & Environmental Engineering, Stanford, United States

P2-03
Evaluation of the vector competence of Dermacentor variabilis (American dog tick) for transmission of Francisella tularensis
S.M. Reese¹, G. Dietrich¹, M.C. Dolan¹, S.W. Sheldon¹, J. Piesman¹, J.M. Petersen¹, R.J. Eisen^1
1Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, United States

P2-05
Isolation of Francisella tularensis from mandibular lymph nodes of red foxes indicates active natural foci in Austria
E. Hofer¹, K. Reisp^1
1Institute for Veterinary Disease Control in Mödling, Austrian Agency for Health and Food Safety (AGES), Mödling, Austria

P2-08
Extra- and intracellular proliferation of Francisella tularensis (LVS) in presence of amoebae
A. Duerrenfeld¹, M. Laue¹, K. Madela¹, R. Grunow^1
1Robert Koch-Institut, Center for Biological Security, Berlin, Germany

P2-09
Application of multiple-locus variable number tandem repeat analysis (MLVA) for subtyping of Swedish isolates of Francisella tularensis
T. Wahab¹, M. Hjertqvist², I. Hedenström³, S. Löfdahl^3
1Swedish Institute for Infectious Disease Control, Centre for Microbiological Preparedness (KCB), Solna, Sweden, ²Swedish Institute for Infectious Disease Control, Department of Epidemiology, Solna, Sweden, ³Swedish Institute for Infectious Disease Control, Department of Bacteriology, Solna, Sweden

P2-10
An outbreak of tularaemia in Tuscany, Central Italy, linked to a natural spring water
M. Fabbi¹, D. Messeri², N. Vicari¹, L. Marino Merlo³, C. Pierozzi³, M. Talini³, G. Perelli⁴, W. Wanderlingh^3
1Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Pavia Department, National Laboratory Reference for Tularemia, Pavia, Italy, ²Pistoia Hospital, Infectious Disease Division, Pistoia, Italy, ³U.S.L. 3 Pistoia, Department of Local Public Health Unit, Pistoia, Italy, ⁴U.S.L.3 Pistoia, Department of Local Veterinary Public Health Unit, Pistoia, Italy

P2-11
Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Chinese Francisella tularensis and its application to some strains
F. Zhang¹, W. Liu², J. He², Q. Duan², W. Cao^2
1The Center for Disease Control and Prevention of Lanzhou Command, LanZhou, China, ²Beijing Institute of Microbiology and Epidemiology, Beijing, China

P2-12
Francisella-like endosymbiont in Dermacentor reticulatus collected in North of Portugal
I. Lopes de Carvalho¹, N. Santos², T. Soares³, L. Zé-Zé¹, M.S. Núncio^1
1Instituto Nacional de Saúde Dr. Ricardo Jorge, Águas de Moura, Portugal, ²Parque Natural Peneda-Gerês, Braga, Portugal, ³Universidade de Évora, Évora, Portugal

P2-13
Longterm follow up of a natural focus of tularemia in south-west Slovakia
V. Výrosteková¹, D. Gurycová¹, L. Madarová², C. Klement^2
1Comenius University, Medical Faculty, Institute of Epidemiology, Bratislava, Slovakia, ²Regional Authority of Public Health, Section of Medical Microbiology, Banská Bystrica, Slovakia

P2-14
The course of tularemia in Kosovo since the first outbreak in 1999
R. Grunow¹, A. Kalaveshi², A. Jenzora¹, G. Mulliqi Osmani², N. Ramadani^2
1Robert Koch-Institut, Center for Biological Security, Berlin, Germany, ²National Institute of Public Health of Kosova, Prishtina, Kosovo, Republic of

P2-15
Epidemiologic situation in the occurrence of tularemia in Slovakia, 1997 - 2008
D. Gurycová¹, V. Výrosteková¹, K. Tináková², E. Gacíková^3
1Comenius University, Medical Faculty, Institute of Epidemiology, Bratislava, Slovakia, ²Regional Authority of Public Health, Section of Epidemiology, Nitra, Slovakia, ³State Veterinary and Food Institute, Department of Serology and Immunology, Bratislava, Slovakia

P2-16
Tularaemia seroprevalence studies under foxes and raccoon dogs in Germany
A. Kuehn¹, C. Schulze², P. Kutzer², H. Ranisch¹, S. Bestek³, H. Nattermann¹, R. Grunow^1
1Robert Koch-Institute, Centre for Biological Safety, Berlin, Germany, ²Landeslabor Berlin-Brandenburg, Frankfurt/Oder, Germany, 3Robert Koch-Institute, Berlin, Germany

P2-17
Monitoring the survival of fish Francisella pathogens in aquatic microcosms
S. Duodu¹, D. Colquhoun^1
1National Veterinary Institute, Fish Health, Oslo, Norway

P2-21
A real-time PCR array for hierarchal identification of environmental and human pathogenic Francisella
K. Svensson^1,2, M. Granberg¹, L. Karlsson¹, V. Neubauerova³, M. Forsman¹, A. Johansson^1,2
1Swedish Defence Research Agency (FOI), CBRN Defence and Security, Umeå, Sweden, ²Umeå University Hospital, Infectious Diseases, Umeå, Sweden, ³Purkyne Military Medical Academy, Hradec Králové, Czech Republic

P2-23
The efficacy of oral vaccination with a defined Francisella vaccine strain in inducing protective immunity against pneumonic tularemia
J.-J. Yu¹, Y. Cong¹, M.N. Guentzel¹, K.E. Klose¹, B.P. Arulanandam^1
1University of Texas at San Antonio, South Texas Center for Emerging Infectious Disease, San Antonio, United States

P2-24
Francisella tularensis suppresses the proinflammatory response of endothelial cells via the endothelial protein C receptor
D.C. Bublitz¹, M.B. Furie¹, J.L. Benach^1
1Stony Brook University, Center for Infectious Diseases, Stony Brook, United States

P2-25
Early immune responses to the Live vaccine strain and SchuS4 infection in cynomolgus macaques
A.B. DuBois¹, J.A. Hutt², T. Wu¹, C.R. Lyons^1
1University of New Mexico, Center for Infectious Disease and Immunity, Albuquerque, United States, ²Lovelace Biomedical and Environmental Research Institute, Albuquerque, United States

P2-26
The pathology of Francisella tularensis SCHU S4 in Cynomolgus macaques
J.A. Hutt¹, M.M. Valderas¹, J.A. Wilder¹, T.L. Brasel¹, R.S. Sherwood¹, C.R. Lyons^2
1Lovelace Respiratory Research Institute, Albuquerque, United States, ²University of New Mexico, Albuquerque, United States

P2-28
T cell Epitope Identification in F. tularensis
J.A. Frelinger¹, M. Valentino², L. Hensley¹, M. Woolard¹, P. McPherson², Z. Maben¹, T. Kawula¹, J.G. Frelinger^2
1University of North Carolina, Microbiology and Immunology, Chapel Hill, United States, ²University of Rochester, Microbiology and Immunology, Rochester, United States

P2-31
Pyrin enhances IL-1ß processing and release in response to Francisella
M.A. Gavrilin¹, S. Mitra¹, S. Seshadri¹, F. Berhe¹, M.D. Wewers^1
1The Ohio State University, Columbus, United States

P2-32
A partial screen for Francisella tularensis virulence determinants using Drosophila melanogaster
M. Åhlund¹, P. Rydén², A. Sjöstedt1, S. Stöven^1
1Umeå University, Clinical Microbiology, Umeå, Sweden, ²Umeå University, Mathematics and Mathematical Statistics, Umeå, Sweden

P2-35
A novel role for Francisella tularensis antioxidant enzymes in modulating human macrophage signaling and activation
A. Melillo¹, J.A. Melendez¹, C.S. Bakshi^1
1Albany Medical College, Center for Immunology and Microbial Disease, Albany, United States

P2-36
A capsule-deficient mutant of Francisella tularensis live vaccine strain is significantly more attenuated than LVS yet induces comparable protection in mice against F. tularensis challenge
Q. Jia¹, B.-Y. Lee¹, M.A. Horwitz^1
1School of Medicine, University of California, Los Angeles, United States

P2-40
Analyses of phagocytic ability of rat macrophages for Francisella tularensis
H. Ray¹, P. Chu¹, J. Yu¹, M.N. Guentzel¹, T. Wu², K.E. Klose¹, B.P. Arulanandam^1
1University of Texas at San Antonio, South Texas Center for Emerging Infectious Disease, San Antonio, United States, ²University of New Mexico Health Science Center, Immunology, Inflammation and Infectious Disease Center, Department of Internal Medicine, Albuquerque, United States

P2-41
Activation of apoptotic pathways in the course of B cell infection with Francisella tularensis
Z. Krocova¹, L. Zivna^2
1FMHS UoD, Inst. Molec. Pathol., Hradec Kralove, Czech Republic, ²FMHS, UoD, Hradec Kralove, Czech Republic

P2-43
Depletion of dendritic cells impairs the innate response to Francisella tularensis LVS
S.A. Roberts¹, K.L. Elkins^1
1FDA, Center for Biologics Evaluation and Research, Bethesda, United States

P2-44
Passive immunization with immunoglobulin G protects against a lethal respiratory infection with type A Francisella tularensis in Fischer 344 rats
G. Mara Koosham¹, J.A. Hutt¹,2, C.R. Lyons¹, T.H. Wu^1
1University of New Mexico School of Medicine, Center for Infectious Disease and Immunity, Albuquerque, United States, ²Lovelace Respiratory Research Institute, Albuquerque, United States

P2-45
Importance of B cells in parenteral murine infection with Francisella novicida
A.Y. Chou¹, K.L. Elkins^1
1FDA, Center for Biologics Evaluation and Research, Bethesda, United States

P2-47
Full virulence is restored by reintroduction of two virulence loci into the live vaccine strain (LVS) of Francisella tularensis
E. Salomonsson^1,2, K. Kuoppa¹, A.-L. Forslund^1,2, C. Zingmark³, I. Golovliov³, A. Sjöstedt³, L. Noppa¹, Å. Forsberg^1,2
1FOI Swedish Defence Research Agency, Department of CBRN Defence and Security, Umeå, Sweden, ²Umeå University, Centre for Microbial Research (UCMR) and Laboratory for Molecular Infection Medicine (MIMS), Department of Molecular Biology, Umeå, Sweden, ³Umeå University, Umeå Centre for Microbial Research (UCMR) and Laboratory for Molecular Infection Medicine (MIMS), Department of Clinical Mirobiology, Umeå, Sweden

P2-48
Advanced phage display – perspective approach for preparing of Francisella tularensis monoclonal antibodies
K. Kubelkova^1,2, S. Balu³, J. Ivanyi³, A. Macela², J. Stulik^2
1University of Defence, Faculty of Military Health Sciences, Centre of Advanced Studies, Hradec Kralove, Czech Republic, ²University of Defence, Faculty of Military Health Sciences, Institute of Molecular Pathology, Hradec Kralove, Czech Republic, ³King´s College London, Guy´s Hospital, Clinical and Diagnostic Sciences Group, London, United Kingdom

P2-49
Optimization of aerosol generation techniques for Francisella tularensis LVS and SchuS4 strains
R.L. Sherwood¹, T. Brasel¹, E. Barr^1
1Lovelace Respiratory Research Institute, Albuquerque, United States

P2-50
Drosophila RNAi screen for host factors required for Francisella tularensis infection
C. Akimana¹, S. Al Khodor¹, Y. Abu Kwaik^1
1University of Louisville, Microbiology and Immunology, Louisville, United States

P2-54
Susceptibility to disease following intraperitoneal infection with Francisella tularensis differs between Fisher 344 and Sprague-Dawley rat strains
C.R. Raymond¹, H. Shen¹, J.W. Conlan^1
1National Research Council of Canada, Institute for Biological Sciences, Ottawa, Canada

P2-60
Virulence comparisons between Schu S4 (A1a) and A1a, A1b, A2 and type B strains using temperature as a surrogate endpoint for death
C.R. Molins¹, M.J. Delorey¹, J.W. Young¹, B.M. Yockey¹, S.M. Reese¹, S.W. Sheldon¹, J.T. Belisle², M.E. Schriefer¹, J.M. Petersen^1
1Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, Colorado, United States, ²Colorado State University, Department of Microbiology, Immunology and Pathology, Fort Collins, Colorado, United States

P2-61
Evaluation of defined Francisella tularensis subsp. tularensis attenuated strains for vaccine efficacy in a murine model
C. Lauriano¹, H. Ray¹, P. Chu¹, J. Yu¹, Y. Cong¹, B. Arulanandam¹, K.E. Klose^1
1University of Texas at San Antonio, South Texas Center for Emerging Infectious Diseases, San Antonio, United States

P2-65
Characterization of a nonhuman primate aerosol exposure system for generation, delivery, and collection of Francisella tularensis DVC SCHU S4
D. Fisher¹, R. Barnewall¹, E. Heller¹, R. Krile¹, A. Lindsay¹, D. Pak¹, G. Sparks¹, J. Price², L.A. Wolfraim^2
1Battelle Biomedical Research Center, West Jefferson, United States, ²DynPort Vaccine Company LLC, Frederick, United States